Thesis offert: Nanoscience in translation

Graduate School: Natural and Engineering Sciences

PhD programme: Nanoscience and Advanced Technologies

Application Deadline: 16 April 2018

Start Date: 1st October 2018

Duration: 36 months

The PhD programme in Nanosciences and Advanced Technologies aims at coordinating a wide research area focussed on nanoscience where the most recent and innovative technologies in physical chemistry are exploited to target translational research with special reference to the biomedical field. An extensive range of scientific expertise, technology, and effort is dedicated to the following objectives: Synthesizing and characterising innovative nanomaterials, Establishing reliable screening procedures to evaluate nanomaterials and nanovectors safety in vitro and in vivo, Assessing nanovectors validity for theranostics with general aim at “Developing biomimetic nanomaterials with active targeting properties and their in vitro and in vivo assay”

Among the main themes for this application area, the applicants should focus on:
•Synthesis of biomimetic polymeric nanoparticles active in recruiting a defined protein corona composition.
•Assay of nanomaterial and nanovectors in cell cultures for toxicity, subcellular targeting and distribution, storage and degradation, and biological effect.
•Assay of nanomaterial and nanovectors in laboratory animals for toxicity, organ targeting and biodistribution, and biological effect.

Research proposals covering the themes above are invited. Priority will be given to multidisciplinary projects.


INFO For further information about the research topics
, please, contact Prof. Franco Tagliaro (franco.tagliaro@univr.it) and Giovanna LOLLO (giovanna.lollo@univ-lyon1.fr)

PhD thesis offer between :
Université Lyon 1, LAGEP, 43 Boulevard du 11 Novembre 1918, Bâtiment CPE
69622 Villeurbanne Cedex, France
and
University of Verona. Dep.Neurosciences, Biomedicine and Movement Sciences,(Italy)

PhD “Design and development of new gene delivery systems for the treatment of autoimmune diseases”

PhD thesis offer
Université Lyon 1, LAGEP, 43 Boulevard du 11 Novembre 1918, Bâtiment CPE
69622 Villeurbanne Cedex, FRANCE

Title: “Design and development of new gene delivery systems for the treatment of autoimmune diseases” – 3 years under the supervision of Prof. Stephanie Briancon and Dr. Giovanna Lollo
Contact : Dr Giovanna Lollo giovanna.lollo@univ-lyon1.fr, Prof. Stephanie Briancon stephanie.briancon@univ-lyon1.fr
Keywords: Nanocarriers, mRNA (messenger ribonucleic acid), biomaterials, lipids, physico-chemical characterization, myeloid derived suppressor cells, in vivo studies
Starting date: April 2018
Laboratory: The LAGEP (Laboratoire d’Automatique et de Génie des Procédés) is a research laboratory of the University Lyon 1 and the French national Institute for scientific research (CNRS UMR 5007). It develops pluridisciplinary research in chemical engineering, pharmaceutical engineering and biochemical processes. The LAGEP gathers strong pluridisciplinary competences in close collaboration with national and international research and development centers in industry or in academia. The “pharmaceutical engineering” group has its strong expertise in drug delivery. The research encompasses several scientific domains as development of new drug delivery systems, physical chemistry properties, biological in vitro evaluation, in vivo biodistribution and efficacy in mice models.

Subject: We are looking for a motivated and pro-active student to be part of an international academic/industrial European project in Gene therapy working closely with high renowned research groups and one of the leader companies in Gene therapy worldwide.
The role of the PhD student will be to design, characterize, produce and evaluate novel nanomedicines for the delivery of genetic material (mRNA) for human applications.
Specific tasks of the student will involve:
i) the design and formulation of intelligent nanoparticulate systems based on either FDA approved materials or new chemical entities to ensure the correct biodistribution of the systems in vivo;
ii) the evaluation of different ingredients on the formulation to warrant the right intracellular activity and delivery of the mRNA;
iii) the modification the selected formulations to provide them targeting properties for tissue/cell specific delivery;
iv) the evaluation of the association, integrity and activity of the encapsulated/associated genetic material.
The student will interact with other students and researchers of the consortia to establish research lines, communicate and discuss the achieved results.
We offer a full industrial/academic training in the formulation and evaluation of nanosystems for drug delivery. Full support on the development of nanoparticles and in vitro evaluation. Training in basics of molecular biology analytics (mRNA quantification, characterization, identification and lab-manipulation) and an international and innovative working group.

Required skills:
- A master degree in Pharmaceutical sciences or Molecular biology.
- Knowledge on genetics or molecular biology is a plus.
- Hands on experience with advance analytical and bioanalytical techniques such as HPLC, electrophoresis, Western blot.
- Hands on experience on cell culture or working under aseptic conditions for the manipulation of the mRNA.
- A very strong chemical background and preferentially experience in conducting small chemical reactions on polymers or particles.
- Basic knowledge of GMP working conditions.
- A very innovative spirit and willing to be part of a multicultural and multidisciplinary environment
- A self-motivated person able to think “out of the box” and having a “problem-solving” approach
- Good presentation skills
- Fluent English and French
Please send to giovanna.lollo@univ-lyon1.fr and stephanie.briancon@univ-lyon1.fr the following documents:
- CV,
- motivation letter,
- two recommendation letters.