PhD thesis offer
Université Lyon 1, LAGEP, 43 Boulevard du 11 Novembre 1918, Bâtiment CPE
69622 Villeurbanne Cedex, FRANCE

Title: “Design and development of new gene delivery systems for the treatment of autoimmune diseases” – 3 years under the supervision of Prof. Stephanie Briancon and Dr. Giovanna Lollo
Contact : Dr Giovanna Lollo giovanna.lollo@univ-lyon1.fr, Prof. Stephanie Briancon stephanie.briancon@univ-lyon1.fr
Keywords: Nanocarriers, mRNA (messenger ribonucleic acid), biomaterials, lipids, physico-chemical characterization, myeloid derived suppressor cells, in vivo studies
Starting date: April 2018
Laboratory: The LAGEP (Laboratoire d’Automatique et de Génie des Procédés) is a research laboratory of the University Lyon 1 and the French national Institute for scientific research (CNRS UMR 5007). It develops pluridisciplinary research in chemical engineering, pharmaceutical engineering and biochemical processes. The LAGEP gathers strong pluridisciplinary competences in close collaboration with national and international research and development centers in industry or in academia. The “pharmaceutical engineering” group has its strong expertise in drug delivery. The research encompasses several scientific domains as development of new drug delivery systems, physical chemistry properties, biological in vitro evaluation, in vivo biodistribution and efficacy in mice models.

Subject: We are looking for a motivated and pro-active student to be part of an international academic/industrial European project in Gene therapy working closely with high renowned research groups and one of the leader companies in Gene therapy worldwide.
The role of the PhD student will be to design, characterize, produce and evaluate novel nanomedicines for the delivery of genetic material (mRNA) for human applications.
Specific tasks of the student will involve:
i) the design and formulation of intelligent nanoparticulate systems based on either FDA approved materials or new chemical entities to ensure the correct biodistribution of the systems in vivo;
ii) the evaluation of different ingredients on the formulation to warrant the right intracellular activity and delivery of the mRNA;
iii) the modification the selected formulations to provide them targeting properties for tissue/cell specific delivery;
iv) the evaluation of the association, integrity and activity of the encapsulated/associated genetic material.
The student will interact with other students and researchers of the consortia to establish research lines, communicate and discuss the achieved results.
We offer a full industrial/academic training in the formulation and evaluation of nanosystems for drug delivery. Full support on the development of nanoparticles and in vitro evaluation. Training in basics of molecular biology analytics (mRNA quantification, characterization, identification and lab-manipulation) and an international and innovative working group.

Required skills:
- A master degree in Pharmaceutical sciences or Molecular biology.
- Knowledge on genetics or molecular biology is a plus.
- Hands on experience with advance analytical and bioanalytical techniques such as HPLC, electrophoresis, Western blot.
- Hands on experience on cell culture or working under aseptic conditions for the manipulation of the mRNA.
- A very strong chemical background and preferentially experience in conducting small chemical reactions on polymers or particles.
- Basic knowledge of GMP working conditions.
- A very innovative spirit and willing to be part of a multicultural and multidisciplinary environment
- A self-motivated person able to think “out of the box” and having a “problem-solving” approach
- Good presentation skills
- Fluent English and French
Please send to giovanna.lollo@univ-lyon1.fr and stephanie.briancon@univ-lyon1.fr the following documents:
- CV,
- motivation letter,
- two recommendation letters.